Difference between revisions of "Part:BBa K3853006"
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<partinfo>BBa_K3853006 short</partinfo> | <partinfo>BBa_K3853006 short</partinfo> | ||
| − | This part is a double-stranded DNA strand designed by ourselves. It can be used with <partinfo>BBa_K3853003</partinfo> and <partinfo>BBa_K3853007</partinfo> to fix the dCas9 protein with sgRNA in a specific position. We use this element as a scaffold for our multi-enzyme complex. This part used as fixed chains for various protein elements | + | This part is a double-stranded DNA strand designed by ourselves. It can be used with <partinfo>BBa_K3853003</partinfo> and <partinfo>BBa_K3853007</partinfo> to fix the dCas9 protein with sgRNA in a specific position. We use this element as a '<b>scaffold</b>' for our multi-enzyme complex. This part used as fixed chains for various protein elements |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 18:29, 12 October 2021
dsDNA
This part is a double-stranded DNA strand designed by ourselves. It can be used with BBa_K3853003 and BBa_K3853007 to fix the dCas9 protein with sgRNA in a specific position. We use this element as a 'scaffold' for our multi-enzyme complex. This part used as fixed chains for various protein elements Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 15
- 1000COMPATIBLE WITH RFC[1000]