Difference between revisions of "Part:BBa K3853004"
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<partinfo>BBa_K3853004 short</partinfo> | <partinfo>BBa_K3853004 short</partinfo> | ||
| − | This part codes for a protein that recognizes and forms a covalent isopeptide bound to a oligopeptide, called SpyTag. That oligopeptide can be found under <partinfo>BBa_K3853005</partinfo>.This part codes the same protein as <partinfo>BBa_K1159200</partinfo>. This is a codon-optimized version to improve its expression in <i>Pichia pastoris</i> GS115. | + | This part codes for a protein that recognizes and forms a covalent isopeptide bound to a oligopeptide, called SpyTag. That oligopeptide can be found under <partinfo>BBa_K3853005</partinfo>. This part codes the same protein as <partinfo>BBa_K1159200</partinfo>. This is a codon-optimized version to improve its expression in <i>Pichia pastoris</i> GS115. |
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Latest revision as of 22:21, 15 October 2021
SpyTag Codon adapted for Pichia pastoris
This part codes for a protein that recognizes and forms a covalent isopeptide bound to a oligopeptide, called SpyTag. That oligopeptide can be found under BBa_K3853005. This part codes the same protein as BBa_K1159200. This is a codon-optimized version to improve its expression in Pichia pastoris GS115.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]