Difference between revisions of "Part:BBa K3776025"

 
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
 +
 +
 +
 +
<html>
 +
<div class="titleWrapper"><div class="titleBackground" style="background-image:none"></div><div class="title"></div></div>
 +
<article>
 +
<style>
 +
.imageContainer1x2 {
 +
display: flex;
 +
flex-wrap: wrap;
 +
justify-content: space-around;
 +
align-items: center;
 +
}
 +
.imageContainer1x2 div {
 +
width: 50%;
 +
margin-bottom: 2%;
 +
text-align:center;
 +
}
 +
.imageContainer1x2 div img {
 +
width: 100%;
 +
}
 +
.imageContainer1x4 {
 +
display: flex;
 +
flex-wrap: wrap;
 +
justify-content: space-around;
 +
align-items: center;
 +
}
 +
.imageContainer1x4 div {
 +
width: 23%;
 +
margin-bottom: 2%;
 +
text-align:center;
 +
}
 +
.imageContainer1x4 div img {
 +
width: 100%;
 +
}
 +
<i>
 +
</style>
 +
</html>
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K3776033 short</partinfo>
+
<partinfo>BBa_K3776025 short</partinfo>
 +
 
 +
<html>
 +
<p align="justify">
 +
 
 +
This TniQ protein is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering (see <a href="https://parts.igem.org/Part:BBa_K3776022"> BBa_K3776022</a>). TniQ forms a complex with the Cas proteins Cas8, Cas7 and Cas6, crucial for DNA binding and insertion.(Klompe et al., 2019)
 +
 
 +
<html>
 +
<p align="justify">
 +
 
  
−
save this
+
</b>
 +
</ul>
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 10: Line 59:
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K3776033 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K3776025 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K3776033 parameters</partinfo>
+
<partinfo>BBa_K3776025 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 10:05, 18 October 2021



TniQ

This TniQ protein is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering (see BBa_K3776022). TniQ forms a complex with the Cas proteins Cas8, Cas7 and Cas6, crucial for DNA binding and insertion.(Klompe et al., 2019)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 828
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]