Difference between revisions of "Part:BBa K3982001:Design"
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− | [https://www.addgene.org/112502 | + | [https://www.addgene.org/112502/ Addgene plasmid # 112502] |
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Latest revision as of 14:50, 21 October 2021
Cas14a1 expression gene
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1357
Illegal BamHI site found at 951 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313
Illegal AgeI site found at 607 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It contains EcoR1 and Pst1 sites. To clone or excise this part, preferably use XBa1 and Spe1 Restriction Enzymes.