Difference between revisions of "Part:BBa K4005001"

 
 
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promoter
 
promoter
 +
=== Profile ===
 +
==== Name: P7 ====
 +
==== Base Pairs: 19bp ====
 +
==== Origin: T7 phage, genome ====
 +
==== Properties: A promoter for initiation of the transcription. ====
 +
 +
=== Construct design ===
 +
[[File:T--Shanghai Metro Utd--BBa K4005001-figure1.png|500px|thumb|center|Figure 1. The rANG protein expression box..]]
 +
The profiles of every basic part are as follows:
 +
 +
=== Proof of function ===
 +
 +
We did SDS-PAGE assey of protein and have obtained Freeze-dried powder of the ANG protein produced by our engineered Escherichia coli (Figure 2).
 +
[[File:T--Shanghai Metro Utd--BBa K4005001-figure2a.png|500px|thumb|center|Figure 2a. SDS-PAGE assey of protein and Freeze-dried powder of the ANG protein..]]
 +
[[File:T--Shanghai Metro Utd--BBa K4005001-figure2b.png|500px|thumb|center|Figure 2b. SDS-PAGE assey of protein and Freeze-dried powder of the ANG protein..]]
 +
 +
=== Reference ===
 +
====1.World Health Organization Estimated Deaths 2012.http://www.who.int/entity/healthinfo/global_burden_disease/GHE_Deaths_2012_country.xls?ua=1====
 +
====2.Biography.com Editors. (2017, April 28). Muhammad Ali. Biography. https://www.biography.com/athlete/muhammad-ali====
 +
====3.Parkinson disease: MedlinePlus genetics. (n.d.). MedlinePlus-Health Information from the National Library of Medicine.https://medlineplus.gov/genetics/condition/parkinson-disease/====
 +
====4.Zhang J, et al. Nucleic Acids Res. 2002 Mar 1;30(5):1169-75====
 +
====5.https://www.ncbi.nlm.nih.gov/Structure/pdb/5EOP====
 +
====6.Sheng J, et al. Acta Biochim Biophys Sin (Shanghai). 2016 May;48(5):399-410. doi: 10.1093/abbs/gmv131.====
 +
====7.Aparicio-Erriu IM, et al. Front Neurosci. 2012 Nov 19;6:167. doi: 10.3389/fnins.2012.00167.====
 +
====8.Wang YN, et al. Cancer Cell. 2018 Apr 9;33(4):752-769.e8. doi: 10.1016/j.ccell.2018.02.012.====
  
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===Usage and Biology===
 
  
 
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Latest revision as of 14:01, 18 October 2021


promoter

promoter

Profile

Name: P7

Base Pairs: 19bp

Origin: T7 phage, genome

Properties: A promoter for initiation of the transcription.

Construct design

Figure 1. The rANG protein expression box..

The profiles of every basic part are as follows:

Proof of function

We did SDS-PAGE assey of protein and have obtained Freeze-dried powder of the ANG protein produced by our engineered Escherichia coli (Figure 2).

Figure 2a. SDS-PAGE assey of protein and Freeze-dried powder of the ANG protein..
Figure 2b. SDS-PAGE assey of protein and Freeze-dried powder of the ANG protein..

Reference

1.World Health Organization Estimated Deaths 2012.http://www.who.int/entity/healthinfo/global_burden_disease/GHE_Deaths_2012_country.xls?ua=1

2.Biography.com Editors. (2017, April 28). Muhammad Ali. Biography. https://www.biography.com/athlete/muhammad-ali

3.Parkinson disease: MedlinePlus genetics. (n.d.). MedlinePlus-Health Information from the National Library of Medicine.https://medlineplus.gov/genetics/condition/parkinson-disease/

4.Zhang J, et al. Nucleic Acids Res. 2002 Mar 1;30(5):1169-75

5.https://www.ncbi.nlm.nih.gov/Structure/pdb/5EOP

6.Sheng J, et al. Acta Biochim Biophys Sin (Shanghai). 2016 May;48(5):399-410. doi: 10.1093/abbs/gmv131.

7.Aparicio-Erriu IM, et al. Front Neurosci. 2012 Nov 19;6:167. doi: 10.3389/fnins.2012.00167.

8.Wang YN, et al. Cancer Cell. 2018 Apr 9;33(4):752-769.e8. doi: 10.1016/j.ccell.2018.02.012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]