Difference between revisions of "Part:BBa K3930032"
 
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
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<p>The SpecR cassette is composed by the promoter PaadA and the resistance gene <i>specR (aadA)</i>. The resistance gene is based on the part (BBa_BBa_K1323012), but it was codon optimized for expression into <i>S.elongatus</i>.</p>
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<p>The SpecR cassette is composed of the promoter pAadA and the resistance gene <i>specR</i> (<i>aadA</i>). The resistance gene is based on part <a href="https://parts.igem.org/Part:BBa_K1323012" class="pr-0" target="_blank">(BBa_K1323012)</a>, but it was codon optimized for expression into <i>S. elongatus</i>.</p>
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<p>This gene codes for a Aminoglycoside adenylyltransferase , which inactivate the spectinomycin by adding a adenyl group on it. Therefore, this cassette aload the selection of Yeast transformant upon addition of spectinomycin into the media of culture. </p>  
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<p>This gene codes for an aminoglycoside adenyltransferase, which inactivates spectinomycin by adding an adenyl group. Therefore, this cassette allows the selection of yeast transformants upon addition of spectinomycin into the media. </p>  
  
 
<h3>Part characterization</h3>
 
<h3>Part characterization</h3>
  
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<p> The part (BBa_K3930032) was used to select the right genomic integration of the part (BBa_K3930026). Transformants were plated onto BG11 + 10 &mu;g.ml-1 spectinomycin Petri dish. The Toulouse INSA UPS team manage to select a transformant with this selection marker (for more details, check the (BBa_K3930026) part page) </p>
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<p> The part (BBa_K3930032) was used to select for genomic integration of part <a href="https://parts.igem.org/Part:BBa_K3930026" class="pr-0" target="_blank">(BBa_K3930026)</a>. Transformants were plated onto BG11 Petri plates containing 10 &mu;g.ml-1 spectinomycin. The Toulouse INSA UPS team managed to select transformants with this selection marker (for more details, check the <a href="https://parts.igem.org/Part:BBa_K3930026" class="pr-0" target="_blank">(BBa_K3930026)</a> part page). </p>
  
 
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Latest revision as of 06:53, 17 October 2021


Spectinomycine selective cassette Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7

Introduction

The SpecR cassette is composed of the promoter pAadA and the resistance gene specR (aadA). The resistance gene is based on part (BBa_K1323012), but it was codon optimized for expression into S. elongatus.

This gene codes for an aminoglycoside adenyltransferase, which inactivates spectinomycin by adding an adenyl group. Therefore, this cassette allows the selection of yeast transformants upon addition of spectinomycin into the media.

Part characterization

The part (BBa_K3930032) was used to select for genomic integration of part (BBa_K3930026). Transformants were plated onto BG11 Petri plates containing 10 μg.ml-1 spectinomycin. The Toulouse INSA UPS team managed to select transformants with this selection marker (for more details, check the (BBa_K3930026) part page).