Difference between revisions of "Part:BBa K3887001"

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=Description=
 
=Description=
 
Streptomyces toxytricini (SttH), is an halogenases used during the conversion of indole.  
 
Streptomyces toxytricini (SttH), is an halogenases used during the conversion of indole.  
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In our experiment, SttH and TnaA are used to convert Trp to 6-Br-Trp, then to 6-Br-indole. The strength of SttH is it shows strict regiospecificity for both bromination and chlorination. This made it suitable for the bromination of indole. However, SttH alone shows low reactivity and exists insoluble form, so it was expressed with Fre to increase the conversion of Trp to 6-Br-Trp.  
+
 
 +
In our experiment, SttH and TnaA are used to convert Trp to 6-Br-Trp, then to 6-Br-indole. The strength of SttH is it shows strict C6 regiospecificity for both bromination and chlorination. This made it suitable for the bromination of indole. However, SttH alone shows low reactivity and exists insoluble form, so it was expressed with Fre to increase the conversion of Trp to 6-Br-Trp.  
  
  

Latest revision as of 07:46, 12 October 2021


SttH

tryptophan 6-halogenase

Description

Streptomyces toxytricini (SttH), is an halogenases used during the conversion of indole.

In our experiment, SttH and TnaA are used to convert Trp to 6-Br-Trp, then to 6-Br-indole. The strength of SttH is it shows strict C6 regiospecificity for both bromination and chlorination. This made it suitable for the bromination of indole. However, SttH alone shows low reactivity and exists insoluble form, so it was expressed with Fre to increase the conversion of Trp to 6-Br-Trp.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 550
    Illegal PstI site found at 337
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 550
    Illegal PstI site found at 337
    Illegal PstI site found at 436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 550
    Illegal XhoI site found at 1081
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 550
    Illegal PstI site found at 337
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 550
    Illegal PstI site found at 337
    Illegal PstI site found at 436
    Illegal NgoMIV site found at 210
    Illegal NgoMIV site found at 468
    Illegal NgoMIV site found at 1171
    Illegal NgoMIV site found at 1437
    Illegal AgeI site found at 356
    Illegal AgeI site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]