Difference between revisions of "Part:BBa K3782002"
| Line 1: | Line 1: | ||
| − | |||
| − | |||
<partinfo>BBa_K3782002 short</partinfo> | <partinfo>BBa_K3782002 short</partinfo> | ||
| − | + | =6xHis-Tag for Purification and Identification of Protein of Interest= | |
| − | + | <p style="margin-right:2.5em;" align="justify"> | |
| − | + | The His-Tag is a commonly used sequence both as a reporter and as a purification tag. Its sequence, consisting of 6 histidine residues, has a very high affinity to nickel(II) ions and is therefore used in affinity purification columns with immobilized nickel ions. The binding of the His-Tag to the nickel ions is not efficient at acidic pH values, and any purification process should therefore be done above pH=7. Imidazole is used to elute the protein from the column by competing with the His tag on binding to the immobilized Nickel ions. | |
| − | < | + | </p> |
| − | + | =Sequence and Features= | |
| − | + | ||
| − | < | + | |
| − | + | ||
<partinfo>BBa_K3782002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3782002 SequenceAndFeatures</partinfo> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
Latest revision as of 15:08, 11 October 2021
6XHis-Tag
6xHis-Tag for Purification and Identification of Protein of Interest
The His-Tag is a commonly used sequence both as a reporter and as a purification tag. Its sequence, consisting of 6 histidine residues, has a very high affinity to nickel(II) ions and is therefore used in affinity purification columns with immobilized nickel ions. The binding of the His-Tag to the nickel ions is not efficient at acidic pH values, and any purification process should therefore be done above pH=7. Imidazole is used to elute the protein from the column by competing with the His tag on binding to the immobilized Nickel ions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]