Difference between revisions of "Part:BBa K3982004:Design"
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===Design Notes=== | ===Design Notes=== | ||
The scaffold region has been designed as referred from Ref. 1. It consists of the tracrRNA and regions of crRNA. The guiding region is the 20 bp spacer sequence which is complementary to the katG gene in Mtb and referred from Ref 2. | The scaffold region has been designed as referred from Ref. 1. It consists of the tracrRNA and regions of crRNA. The guiding region is the 20 bp spacer sequence which is complementary to the katG gene in Mtb and referred from Ref 2. | ||
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| + | The 20bp sequence is as follows: gcgatcaccagcggcatcga | ||
===Source=== | ===Source=== | ||
Latest revision as of 12:02, 11 October 2021
CODE M sgRNA for targeting wildtype katG gene in Mycobacterium tuberculosis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The scaffold region has been designed as referred from Ref. 1. It consists of the tracrRNA and regions of crRNA. The guiding region is the 20 bp spacer sequence which is complementary to the katG gene in Mtb and referred from Ref 2.
The 20bp sequence is as follows: gcgatcaccagcggcatcga
Source
References
1) Kim, D.Y., Lee, J.M., Moon, S.B. et al. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Nat Biotechnol (2021). https://doi.org/10.1038/s41587-021-01009-z
2) NCBI Reference Sequence for Mycobacterium tuberculosis H37Rv: NC_000962.3