Difference between revisions of "Part:BBa K3896001"
 
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This part is the coding gene of human aminopeptidase N (hAPN) recombinant protein and PmrB protein in bacterial PmrA / PmrB two-component system.
 
This part is the coding gene of human aminopeptidase N (hAPN) recombinant protein and PmrB protein in bacterial PmrA / PmrB two-component system.
  
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We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of hAPN protein Ala281 to Gly330 to receive the stimulation of S protein of 229E-CoV. After detecting the S protein of 229E-CoV, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA.
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We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of hAPN protein Ala281 to Gly330 to receive the stimulation of S protein of HCoV-229E. After detecting the S protein of HCoV-229E, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA.
  
 
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Latest revision as of 15:09, 20 October 2021


The coding gene of HAPN-PmrB recombinant protein

This part is the coding gene of human aminopeptidase N (hAPN) recombinant protein and PmrB protein in bacterial PmrA / PmrB two-component system.

We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of hAPN protein Ala281 to Gly330 to receive the stimulation of S protein of HCoV-229E. After detecting the S protein of HCoV-229E, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 96
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1081
  • 1000
    COMPATIBLE WITH RFC[1000]