Difference between revisions of "Part:BBa K3731007"

 
 
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<partinfo>BBa_K3731007 short</partinfo>
 
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This composite part is the combination of vgb gene and mazE, which are helpful for the growth of bacteria.
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This composite part is the combination of vgb and mazE gene, which are helpful for the growth of bacteria.
  
 
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<partinfo>BBa_K3731007 parameters</partinfo>
 
<partinfo>BBa_K3731007 parameters</partinfo>
 
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==iGEM2021_Nanjing-China Experiment==
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<div>
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<b>Group: Nanjing-China 2021</b>
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<br>
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<b>Author: Hao Yin</b>
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<br><br>
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<p>Besides, the structure of mazE antitoxin is drawn by PyMol as is shown below.
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[[File:T--Nanjing-China--mazE.png|800px|thumb|center|Figure1 Structure of mazE]]
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The mazE protein is such a useful antitoxin that it can help bacteria grow longer and survive harsher environment, thus producing more polyP. Thus, we insert mazE gene into E. coli genome to increase the productivity.
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</p>
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<p>
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Bacterial hemoglobin (VtHb) is produced by the gram-negative bacterium, Vitreoscilla , in large quantity in response to hypoxic environmental conditions. The vgb gene coding for VtHb has been cloned in E. coli where it is expressed strongly by its natural promoter. The expression of the vgb gene in Vitreoscilla is transcriptionally regulated by oxygen. Vitreoscillu hemoglobin (VHb) is expressed at elevated levels in Vitreoscillu under oxygen-limited cultivation conditions.
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[[File:T--Nanjing-China--vgb.png|800px|thumb|center|Figure1 vgb gene expression and hemoglobin synthesis in response to oxygen level]]
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</p>
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<p>Adding vgb and mazE into the gene helps the growth of bacteria, thus improving the productivity of polyP.  </p>
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<p>Reference:
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Zorzini, Buts et al. Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding, Nucleic Acids Research, Volume 43, Issue 2, 30 January 2015, Pages 1241–1256
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</p>
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<p>Kallio, P. T.; Kim, D.; Tsai, P. S.; Bailey, J. E. Intracellular expression of Vitreoscilla hemoglobin
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alters Escherichia coli energy metabolism under oxygen-limited conditions. The FEBS Journal
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1994, 219 (1-2), 201-208.</p>
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<p>Kanak L. Dikshit, Rajendra P. Dikshit, Dale A. Webster, Study of Vitreoscilla globin(vgb) gene expression and promoter activity in E. Coli through transcriptional fusion , Nucleic Acids Research, Volume 18, Issue 14, 25 July 1990, Pages 4149–4155.</p>

Latest revision as of 13:15, 15 October 2021


vgb+mazE

This composite part is the combination of vgb and mazE gene, which are helpful for the growth of bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


iGEM2021_Nanjing-China Experiment

Group: Nanjing-China 2021
Author: Hao Yin

Besides, the structure of mazE antitoxin is drawn by PyMol as is shown below.

Figure1 Structure of mazE

The mazE protein is such a useful antitoxin that it can help bacteria grow longer and survive harsher environment, thus producing more polyP. Thus, we insert mazE gene into E. coli genome to increase the productivity.

Bacterial hemoglobin (VtHb) is produced by the gram-negative bacterium, Vitreoscilla , in large quantity in response to hypoxic environmental conditions. The vgb gene coding for VtHb has been cloned in E. coli where it is expressed strongly by its natural promoter. The expression of the vgb gene in Vitreoscilla is transcriptionally regulated by oxygen. Vitreoscillu hemoglobin (VHb) is expressed at elevated levels in Vitreoscillu under oxygen-limited cultivation conditions.

Figure1 vgb gene expression and hemoglobin synthesis in response to oxygen level

Adding vgb and mazE into the gene helps the growth of bacteria, thus improving the productivity of polyP.


Reference: Zorzini, Buts et al. Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding, Nucleic Acids Research, Volume 43, Issue 2, 30 January 2015, Pages 1241–1256

Kallio, P. T.; Kim, D.; Tsai, P. S.; Bailey, J. E. Intracellular expression of Vitreoscilla hemoglobin alters Escherichia coli energy metabolism under oxygen-limited conditions. The FEBS Journal 1994, 219 (1-2), 201-208.

Kanak L. Dikshit, Rajendra P. Dikshit, Dale A. Webster, Study of Vitreoscilla globin(vgb) gene expression and promoter activity in E. Coli through transcriptional fusion , Nucleic Acids Research, Volume 18, Issue 14, 25 July 1990, Pages 4149–4155.