Difference between revisions of "Part:BBa K3726080"
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<partinfo>BBa_K3726080 short</partinfo> | <partinfo>BBa_K3726080 short</partinfo> | ||
| − | This part corresponds to a strong constitutive promoter for cyanobacteria. The promoter sequence corresponds with a modified version of the native | + | This part corresponds to a strong constitutive promoter for cyanobacteria. The promoter sequence corresponds with a modified version of the native <i>PCC11801</i> phycocyanin β-1 subunit promoter. |
| − | Despite being a cyanobacterial promoter, it has shown high expression levels also in E. Coli. | + | Despite being a cyanobacterial promoter, it has shown high expression levels also in <i>E. Coli</i>. |
The promoter was firstly documented by P. Wangikar et al. ("A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152.), obtained via the screening of a library of promoter variants created by error-prone PCR. | The promoter was firstly documented by P. Wangikar et al. ("A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152.), obtained via the screening of a library of promoter variants created by error-prone PCR. | ||
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. | The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. | ||
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| + | <b> Contribution from MADRID_UCM </b> | ||
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| + | Group: MADRID_UCM (4C_Fuels Project) | ||
| + | Author: Jorge Fernández, Alvaro Luego, Santiago Barragán Ariza, Lucia Tapia Peña. | ||
| + | Summary: Promoter Prbcl-m36 have demonstrated one of the highest expression levels in <i>Synechococcus elongatus genre </i> as well as related cyanobacteria species. This promoter element has been adapted to Phytobricks standard and succesfully assembled. | ||
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| + | This phytobrick compatible promoter is part of a wider collection of regulatory elements for cyanobacteria, which are part of MADRID_UCM 2021 team contribution to iGEM community. | ||
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Latest revision as of 23:07, 21 October 2021
P_PrbcL-m36
This part corresponds to a strong constitutive promoter for cyanobacteria. The promoter sequence corresponds with a modified version of the native PCC11801 phycocyanin β-1 subunit promoter.
Despite being a cyanobacterial promoter, it has shown high expression levels also in E. Coli.
The promoter was firstly documented by P. Wangikar et al. ("A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152.), obtained via the screening of a library of promoter variants created by error-prone PCR.
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001.
Contribution from MADRID_UCM
Group: MADRID_UCM (4C_Fuels Project) Author: Jorge Fernández, Alvaro Luego, Santiago Barragán Ariza, Lucia Tapia Peña. Summary: Promoter Prbcl-m36 have demonstrated one of the highest expression levels in Synechococcus elongatus genre as well as related cyanobacteria species. This promoter element has been adapted to Phytobricks standard and succesfully assembled.
This phytobrick compatible promoter is part of a wider collection of regulatory elements for cyanobacteria, which are part of MADRID_UCM 2021 team contribution to iGEM community.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 225
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 225
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 225
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 225
- 1000COMPATIBLE WITH RFC[1000]