Difference between revisions of "Part:BBa K3726088:Design"
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===Source=== | ===Source=== | ||
Coding sequence of this RBS has been calculated using DeNovo DNA Software. | Coding sequence of this RBS has been calculated using DeNovo DNA Software. | ||
| − | |||
| + | To know more about Marburg collection guidelines visit https://2018.igem.org/Team:Marburg/Part_Collection | ||
===References=== | ===References=== | ||
A. Reis and H. Salis, "An Automated Model Test System for Systematic Development and Improvement of Gene Expression Models", ACS Synthetic Biology, vol. 9, no. 11, pp. 3145-3156, 2020. | A. Reis and H. Salis, "An Automated Model Test System for Systematic Development and Improvement of Gene Expression Models", ACS Synthetic Biology, vol. 9, no. 11, pp. 3145-3156, 2020. | ||
Latest revision as of 17:36, 8 October 2021
RBS_NphT7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The RBS has been calculated using DeNovo DNA Software. Sequence has been designing including the resulting MoClo Overhangs after level 1 assembly. In accordance with the design of Marburg Collection https://2018.igem.org/Team:Marburg/Part_Collection, the bases TACTAGAG in the 5’ end, and the bases TAATCAATG are part of the RBS sequence.
Source
Coding sequence of this RBS has been calculated using DeNovo DNA Software.
To know more about Marburg collection guidelines visit https://2018.igem.org/Team:Marburg/Part_Collection
References
A. Reis and H. Salis, "An Automated Model Test System for Systematic Development and Improvement of Gene Expression Models", ACS Synthetic Biology, vol. 9, no. 11, pp. 3145-3156, 2020.