Difference between revisions of "Part:BBa K3726093"
 
(3 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3726093 short</partinfo>
 
<partinfo>BBa_K3726093 short</partinfo>
  
−
This part corresponds with an Ribosome Binding SIte sequence specifically designed for the cyanobacteria Synechocystis PCC6803.  This RBS corresponds with a SD consensus sequence for PCC 6803 allowing up to 5-fold higher expression than the typical E. coli AGGAGG consensus sequence.  
+
This part corresponds with an Ribosome Binding SIte sequence specifically designed for the cyanobacteria <i>Synechocystis PCC6803</i>.  This RBS corresponds with a SD consensus sequence for PCC 6803 allowing up to 5-fold higher expression than the typical <i>E. coli</i> AGGAGG consensus sequence.  
  
 
The part has been assembled in accordance with the design guidelines of Marburg Collection https://2019.igem.org/Team:Marburg/Part_Collection.
 
The part has been assembled in accordance with the design guidelines of Marburg Collection https://2019.igem.org/Team:Marburg/Part_Collection.
Line 9: Line 9:
 
Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG  in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence.
 
Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG  in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence.
  
−
The RBS was designed via an analysis of the RBS sequences of all coding genes longer than  100 amino acids in Synechocystis PCC 6803. It was found that only 26% of the genes contained the core SD sequence, while in E. coli it was 57% (Ma et al., 2002). Based on that the consensu RBS sequence TAGTGGAGGT which is complementary to the anti-SD sequence AUCACCUCCUUU of Synechocystis PCC 6803 has been designed. 
 
−
 
−
Fuentes: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181335/
 
−
https://www.sciencedirect.com/science/article/abs/pii/B978012385075100024X?via%3Dihub
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 18: Line 14:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><b>Sequence & Features</b></span>
 
<partinfo>BBa_K3726093 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3726093 SequenceAndFeatures</partinfo>
  

Latest revision as of 15:43, 8 October 2021


RBS*

This part corresponds with an Ribosome Binding SIte sequence specifically designed for the cyanobacteria Synechocystis PCC6803. This RBS corresponds with a SD consensus sequence for PCC 6803 allowing up to 5-fold higher expression than the typical E. coli AGGAGG consensus sequence.

The part has been assembled in accordance with the design guidelines of Marburg Collection https://2019.igem.org/Team:Marburg/Part_Collection.

Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence.


Sequence & Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]