Difference between revisions of "Part:BBa K3726093"
 
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<partinfo>BBa_K3726093 short</partinfo>
 
<partinfo>BBa_K3726093 short</partinfo>
  
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This part corresponds with an Ribosome Binding SIte sequence specifically designed for the cyanobacteria Synechocystis PCC6803.  This RBS corresponds with a SD consensus sequence for PCC 6803 allowing up to 5-fold higher expression than the typical E. coli AGGAGG consensus sequence.  
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This part corresponds with an Ribosome Binding SIte sequence specifically designed for the cyanobacteria <i>Synechocystis PCC6803</i>.  This RBS corresponds with a SD consensus sequence for PCC 6803 allowing up to 5-fold higher expression than the typical <i>E. coli</i> AGGAGG consensus sequence.  
  
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The part has been assembled in accordance with the design guidelines of Marburg Collection https://2019.igem.org/Team:Marburg/Part_Collection. Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG  in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence.
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The part has been assembled in accordance with the design guidelines of Marburg Collection https://2019.igem.org/Team:Marburg/Part_Collection.
  
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The RBS was designed via an analysis of the RBS sequences of all coding genes longer than  100 amino acids in Synechocystis PCC 6803. It was found that only 26% of the genes contained the core SD sequence, while in E. coli it was 57% (Ma et al., 2002). Based on that the consensu RBS sequence TAGTGGAGGT which is complementary to the anti-SD sequence AUCACCUCCUUU of Synechocystis PCC 6803 has been designed.
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Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG  in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence.
  
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Fuentes: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181335/
 
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https://www.sciencedirect.com/science/article/abs/pii/B978012385075100024X?via%3Dihub
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence & Features</b></span>
 
<partinfo>BBa_K3726093 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3726093 SequenceAndFeatures</partinfo>
  

Latest revision as of 15:43, 8 October 2021


RBS*

This part corresponds with an Ribosome Binding SIte sequence specifically designed for the cyanobacteria Synechocystis PCC6803. This RBS corresponds with a SD consensus sequence for PCC 6803 allowing up to 5-fold higher expression than the typical E. coli AGGAGG consensus sequence.

The part has been assembled in accordance with the design guidelines of Marburg Collection https://2019.igem.org/Team:Marburg/Part_Collection.

Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence.


Sequence & Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]