Difference between revisions of "Part:BBa K3726093"

Latest revision as of 15:43, 8 October 2021

RBS*

This part corresponds with an Ribosome Binding SIte sequence specifically designed for the cyanobacteria Synechocystis PCC6803. This RBS corresponds with a SD consensus sequence for PCC 6803 allowing up to 5-fold higher expression than the typical E. coli AGGAGG consensus sequence.

The part has been assembled in accordance with the design guidelines of Marburg Collection https://2019.igem.org/Team:Marburg/Part_Collection.

Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence.

Sequence & Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3726093 short</partinfo>
-z
+This part corresponds with an Ribosome Binding SIte sequence specifically designed for the cyanobacteria <i>Synechocystis PCC6803</i>.  This RBS corresponds with a SD consensus sequence for PCC 6803 allowing up to 5-fold higher expression than the typical <i>E. coli</i> AGGAGG consensus sequence.
+The part has been assembled in accordance with the design guidelines of Marburg Collection https://2019.igem.org/Team:Marburg/Part_Collection.
+Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG   in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence.
 <!-- Add more about the biology of this part here
@@ Line 9: / Line 14: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'><b>Sequence & Features</b></span>
 <partinfo>BBa_K3726093 SequenceAndFeatures</partinfo>