Difference between revisions of "Part:BBa K3930031"

 
 
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<partinfo>BBa_K3930031 short</partinfo>
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3930031 SequenceAndFeatures</partinfo>
  
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This sequence codes for the enzyme the 9-Hydroperoxide lyase that transforms hydroperoxyde-linoleic and hydroperoxyde-linolenic acids into their hydrperoxyde forms. This sequence is codon optimized for an expression into S.elongatus. The 9-HPL sequence comes from Cucumis melo.
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<h2>Introduction</h2>
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<p>This sequence codes for the 9-Hydroperoxide lyase which transforms the hydroperoxyde forms of linoleic and linolenic acids into 3,6-nonadienal. This sequence is codon optimized for expression into <i>S.elongatus</i>. The 9-HPL sequence comes from <i>Cucumis melo</i> and is described in the publication of Vincenti et al, (2019).</p>
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<br>
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<h2>Testing</h2>
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<p>The Toulouse team could not test this part for reasons of time, and preferred to focus on the parts characterization that allow the production of molecules responsible for the floral aspect of the violet perfume.</p>
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<h2>References</h2>
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    <i>
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    <li>Vincenti S, Mariani M, Alberti J-C, Jacopini S, Brunini-Bronzini de Caraffa V, Berti L, Maury J. 2019. Biocatalytic Synthesis of Natural Green Leaf Volatiles Using the Lipoxygenase Metabolic Pathway. Catalysts. 9(10):873. doi:10.3390/catal9100873.</li>
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3930031 SequenceAndFeatures</partinfo>
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Latest revision as of 18:13, 16 October 2021


Gene coding for the 9-Hydroperoxide lyase Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 18
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1012

Introduction

This sequence codes for the 9-Hydroperoxide lyase which transforms the hydroperoxyde forms of linoleic and linolenic acids into 3,6-nonadienal. This sequence is codon optimized for expression into S.elongatus. The 9-HPL sequence comes from Cucumis melo and is described in the publication of Vincenti et al, (2019).


Testing

The Toulouse team could not test this part for reasons of time, and preferred to focus on the parts characterization that allow the production of molecules responsible for the floral aspect of the violet perfume.

References

  1. Vincenti S, Mariani M, Alberti J-C, Jacopini S, Brunini-Bronzini de Caraffa V, Berti L, Maury J. 2019. Biocatalytic Synthesis of Natural Green Leaf Volatiles Using the Lipoxygenase Metabolic Pathway. Catalysts. 9(10):873. doi:10.3390/catal9100873.
    2.