Difference between revisions of "Part:BBa K3930029"

 
 
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<partinfo>BBa_K3930029 short</partinfo>
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3930029 SequenceAndFeatures</partinfo>
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The promoter Trc-Theo is an inducible promoter upon addition of IPTG into the media of culture. moreover there is a translation control due to riboswitch which can be activated by adding theophyline in the media of culture.  
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<h2>Introduction</h2>
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<p>The promoter pTrc-riboswitch E comes from the publication of Nakahira et al. (2013). It comprises a double regulation of the downstream gene expression. The first is a transcriptional regulation by the IPTG-inducible TRC promoter. The second regulation is post-transcriptional, as a theophylline-inducible aptamer sequesters the RBS essential for mRNA translation. This promoter has been originally described for control of gene expression in the cyanobacterium  <i>Synechococcus elongatus</i> PCC 7942 .</p>
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<h2>Testing</h2>
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<p>The Toulouse team could not test this part for reasons of time, and preferred to focus on the parts characterization that allow the production of molecules responsible for the floral aspect of the violet perfume.</p>
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<h2>References</h2>
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    <li>Nakahira Y, Ogawa A, Asano H, Oyama T, Tozawa Y. 2013. Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942. Plant and Cell Physiology. 54(10):1724–1735. doi:10.1093/pcp/pct115.</li>
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<span class='h3bb'>Sequence and Features</span>
 
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Latest revision as of 06:50, 17 October 2021


IPTG and theophylline inducible promoter Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

The promoter pTrc-riboswitch E comes from the publication of Nakahira et al. (2013). It comprises a double regulation of the downstream gene expression. The first is a transcriptional regulation by the IPTG-inducible TRC promoter. The second regulation is post-transcriptional, as a theophylline-inducible aptamer sequesters the RBS essential for mRNA translation. This promoter has been originally described for control of gene expression in the cyanobacterium Synechococcus elongatus PCC 7942 .


Testing

The Toulouse team could not test this part for reasons of time, and preferred to focus on the parts characterization that allow the production of molecules responsible for the floral aspect of the violet perfume.

References

  1. Nakahira Y, Ogawa A, Asano H, Oyama T, Tozawa Y. 2013. Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942. Plant and Cell Physiology. 54(10):1724–1735. doi:10.1093/pcp/pct115.
    2.