Difference between revisions of "Part:BBa K3889110"

 
 
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<partinfo>BBa_K3889110 short</partinfo>
 
<partinfo>BBa_K3889110 short</partinfo>
  
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Constitutive expression cassette for production and detection of xylR repressor for Bacillus subtilis.
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This gene cassette has been designed to be a part of the genetic circuit for an inducible kill switch in <i>Bacillus subtilis</i>. The gene coding for the XylR repressor ( protein is downstream to the P43 constitutive promoter of <i>Bacillus subtilis</i> (<partinfo>BBa_K143013</partinfo>) and RBS for <i>Bacillus subtilis</i> for constitutive expression.
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The xylR gene is upstream to porcine teschovirus-1 P2A Peptide Linker for effective transcription. Downstream to the linker, a reporter gene sfGFP (<partinfo>BBa_K3889002</partinfo>) has been placed to check the working of the cassette in <i>Bacillus subtilis</i>. The cassette is complete with the double terminator (<partinfo>BBa_B0015</partinfo>).
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The cassette has been designed to be a part of a genetic circuit of a xylose inducible kill switch. The constitutive expression of the XylR repressor will suppress the production of the toxin in absence of xylose.
  
 
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Latest revision as of 22:45, 19 October 2021


XylR Repressor Cassette for Bacillus subtilis

This gene cassette has been designed to be a part of the genetic circuit for an inducible kill switch in Bacillus subtilis. The gene coding for the XylR repressor ( protein is downstream to the P43 constitutive promoter of Bacillus subtilis (BBa_K143013) and RBS for Bacillus subtilis for constitutive expression. The xylR gene is upstream to porcine teschovirus-1 P2A Peptide Linker for effective transcription. Downstream to the linker, a reporter gene sfGFP (BBa_K3889002) has been placed to check the working of the cassette in Bacillus subtilis. The cassette is complete with the double terminator (BBa_B0015).

The cassette has been designed to be a part of a genetic circuit of a xylose inducible kill switch. The constitutive expression of the XylR repressor will suppress the production of the toxin in absence of xylose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1196