Difference between revisions of "Part:BBa K3896011"
 
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ACE2 RBD
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The target DNA can express a protein containing 194 amino acids, which is a segment of SARS-CoV-2 RBD, including Thr333 to Gly526. It is a key part of binding to receptor ACE2. The C-terminal has his tag, which is convenient for subsequent protein extraction and purification. The purified protein can specifically bind to pmrCAB (ACE2) to trigger downstream reaction.
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<img src="https://2021.igem.org/wiki/images/5/53/T--NEU_CHINA--testP2.jpg" class="zon4-50p">
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 08:21, 9 October 2021


SARS-CoV-2 RBD with His-tag

The target DNA can express a protein containing 194 amino acids, which is a segment of SARS-CoV-2 RBD, including Thr333 to Gly526. It is a key part of binding to receptor ACE2. The C-terminal has his tag, which is convenient for subsequent protein extraction and purification. The purified protein can specifically bind to pmrCAB (ACE2) to trigger downstream reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 484
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 484
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 610
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 484
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 484
    Illegal NgoMIV site found at 433
  • 1000
    COMPATIBLE WITH RFC[1000]