Difference between revisions of "Part:BBa K3970025"
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==Usage and Biology== | ==Usage and Biology== | ||
| − | + | Gene PcyA encodes ferredoxin oxidoreductase, which catalyzes the four-electron reduction of biliverdin IX-alpha (2-electron reduction at both the A and D rings) and then form (2R,3Z)-phycocyanobilin: | |
| + | <p style="color:blue;">biliverdin IX-alpha + 4 H+ + 4 reduced [2Fe-2S]-[ferredoxin] = biliverdin IX-alpha + 4 oxidized [2Fe-2S]-[ferredoxin]</p> | ||
==Construction== | ==Construction== | ||
| − | + | [[File:0025-1.png|600px|thumb|center]] | |
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==Experiment results== | ==Experiment results== | ||
| − | + | ( note that how we recycle the GAP promoter and ADH1 terminator has been shown on the last parts. And the sequence pcyA has already been synthesized on the plasmid pTDH3 so we don’t have to recycle it.) | |
| − | ( note that how we recycle the GAP promoter and | + | |
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| − | + | ==Sequence== | |
| − | < | + | <p style="word-wrap:break-word;">tcattatcaatactgccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattagccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatatataacatcgtaggtgtctgggtgaacagtttattcctggcatccactaaatataatggagcccgctttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacagagaacaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcctggagtaaatgatgacacaaggcaattgacccacgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctacttgactaataagtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccaagaacttagtttcgaataaacacacataaacaaacaaaagatctgatatggatcgaattagatctcgccaccatgccatgtagaaagttgtgtgatgttgaattaggttcagtcttgatgtctttgagacaacaacaacacccattaatccaaagactggctgatagaatcgaacaaatctggcaaacccacttgcaattagccccatacgaattgccagcagacttgggttacgtcgaaggcaagttggagggtgaaagattggttattgaaaaccattgttaccaagctcctccattcagaaagcttcacctagaattggctcgtgttggtaatactttggatattctacattgcgttatgttcccacaaccaacttacgacttgccaatgtttggttgtgacttggtcggtggtagaggtcaaatctctgctgccattgtcgatttatctccagttaccggtgaattgccaccagcttacaccactgctttgaacgctttaccaaagatcgccttctcccaaaccagagaattgccaccatggggttacatcttctctcctttctgtttgttcattagaccacaaggtgcttccgaagaacagcaattcttggatcgtatcggtgactacttggccttgcactgtcaattgtctcaacaatccaccccattgttggacccacaaccagttattgctggtcaacaacaatactgtcaacaacaacaacaaaacgacaaaactcgtagagttttggaaaaggctttcggtcacgactgggctgaaagatatatgactactgtcttgtttgatgctccatgagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccgg</p> |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 15:01, 14 October 2021
pGAP-PcyA-tADH1
Usage and Biology
Gene PcyA encodes ferredoxin oxidoreductase, which catalyzes the four-electron reduction of biliverdin IX-alpha (2-electron reduction at both the A and D rings) and then form (2R,3Z)-phycocyanobilin:
biliverdin IX-alpha + 4 H+ + 4 reduced [2Fe-2S]-[ferredoxin] = biliverdin IX-alpha + 4 oxidized [2Fe-2S]-[ferredoxin]
Construction
Experiment results
( note that how we recycle the GAP promoter and ADH1 terminator has been shown on the last parts. And the sequence pcyA has already been synthesized on the plasmid pTDH3 so we don’t have to recycle it.)
Sequence
tcattatcaatactgccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattagccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatatataacatcgtaggtgtctgggtgaacagtttattcctggcatccactaaatataatggagcccgctttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacagagaacaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcctggagtaaatgatgacacaaggcaattgacccacgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctacttgactaataagtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccaagaacttagtttcgaataaacacacataaacaaacaaaagatctgatatggatcgaattagatctcgccaccatgccatgtagaaagttgtgtgatgttgaattaggttcagtcttgatgtctttgagacaacaacaacacccattaatccaaagactggctgatagaatcgaacaaatctggcaaacccacttgcaattagccccatacgaattgccagcagacttgggttacgtcgaaggcaagttggagggtgaaagattggttattgaaaaccattgttaccaagctcctccattcagaaagcttcacctagaattggctcgtgttggtaatactttggatattctacattgcgttatgttcccacaaccaacttacgacttgccaatgtttggttgtgacttggtcggtggtagaggtcaaatctctgctgccattgtcgatttatctccagttaccggtgaattgccaccagcttacaccactgctttgaacgctttaccaaagatcgccttctcccaaaccagagaattgccaccatggggttacatcttctctcctttctgtttgttcattagaccacaaggtgcttccgaagaacagcaattcttggatcgtatcggtgactacttggccttgcactgtcaattgtctcaacaatccaccccattgttggacccacaaccagttattgctggtcaacaacaatactgtcaacaacaacaacaaaacgacaaaactcgtagagttttggaaaaggctttcggtcacgactgggctgaaagatatatgactactgtcttgtttgatgctccatgagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccgg
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]