Difference between revisions of "Part:BBa K3726095"

 
 
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__NOTOC__
 
__NOTOC__
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{|width='80%'
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<partinfo>Bba_K3726094 short</partinfo>
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|width=35% valign='top'|
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[[Image:Terminator.png]]
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<partinfo>BBa_K3726095 parameters</partinfo>
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| width=50% valign='top' style='border: 1px solid black'|
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<partinfo>BBa_K3726095 short</partinfo>
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* bxc
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|}
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The sequence of this parts corresponds with a rho-independent (intrinsec) terminator with a proved 99.9% terminating efficiency in UTEX 2973 either in optimal and suboptimal growth conditions.
  
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'''Secondary Structure'''
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The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.
  
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[[Image:Mfold-K3726095-1.png]]
 
  
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<hr>
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'''Measurement'''
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<!-- Add more about the biology of this part here
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'><b>Sequence & Features</b></span>
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<partinfo>BBa_K3726094 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3726094 parameters</partinfo>
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Latest revision as of 17:24, 7 October 2021


T(5a)_Teck

The sequence of this parts corresponds with a rho-independent (intrinsec) terminator with a proved 99.9% terminating efficiency in UTEX 2973 either in optimal and suboptimal growth conditions.

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.


Sequence & Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]