Difference between revisions of "Part:BBa K3875030"

 
 
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===Usage and Biology===
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<strong>Phyb-mazE-T1<br></strong>
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<br>
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<strong>Biology</strong>:<br>
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===Usage and Biology===
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<strong>Phyb  :the promoter of the hyb operon</strong><br>
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<strong>mazE :antitoxic gene</strong><br>
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<br>
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<br>
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<strong>Design:<strong><br>
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We connect MazE with the <i>phyb</i> promoter to ensure that the <i>mazE</i> protein is expressed only under anaerobic conditions, and once the engineered bacteria are exposed to oxygen, drastic changes in oxygen concentration will cause the <i>phyb</i> promoter expression to fail, thus preventing the expression of antitoxins, which in turn will lead to the death of the engineering bacteria<br>
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<br>
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<strong>Reference:</strong><br>
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Richard, D. J., Sawers, G., Sargent, F., McWalter, L., & Boxer, D. H. (1999). Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology (Reading, England), 145 ( Pt 10), 2903–2912.
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https://doi.org/10.1099/00221287-145-10-2903
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Latest revision as of 09:20, 18 October 2021


Usage and Biology

Phyb-mazE-T1

Biology:
Phyb :the promoter of the hyb operon
mazE :antitoxic gene


Design:
We connect MazE with the phyb promoter to ensure that the mazE protein is expressed only under anaerobic conditions, and once the engineered bacteria are exposed to oxygen, drastic changes in oxygen concentration will cause the phyb promoter expression to fail, thus preventing the expression of antitoxins, which in turn will lead to the death of the engineering bacteria


Reference:
Richard, D. J., Sawers, G., Sargent, F., McWalter, L., & Boxer, D. H. (1999). Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology (Reading, England), 145 ( Pt 10), 2903–2912. https://doi.org/10.1099/00221287-145-10-2903

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]