Difference between revisions of "Part:BBa K3726021:Design"

 
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===Design Notes===
 
===Design Notes===
 
This part corresponds with the codon optimized coding sequence of PKPa.
 
This part corresponds with the codon optimized coding sequence of PKPa.
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Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942.
+
Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>  .
 
In addition the part sequence has been optimized to improve  mRNA stability removing internal recognition sites for endonucleases
 
In addition the part sequence has been optimized to improve  mRNA stability removing internal recognition sites for endonucleases
−
 
−
 
−
 
  
 
===Source===
 
===Source===
  
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Coding sequence of this Xylulose-5-phosphate phosphoketolase has been found within the genome of Pseudomonas aeruginosa.
+
Coding sequence of this Xylulose-5-phosphate phosphoketolase has been found within the genome of <i>Pseudomonas aeruginosa</i>.
 
Uniprot reference: https://www.uniprot.org/uniprot/A0A2R4KQ27
 
Uniprot reference: https://www.uniprot.org/uniprot/A0A2R4KQ27
  
 +
===References===
  
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===References===
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W. Xiong et al., "Phosphoketolase pathway contributes to carbon metabolism in cyanobacteria", 2021.
 +
 
 +
I. Bogorad, T. Lin and J. Liao, "Synthetic non-oxidative glycolysis enables complete carbon conservation", 2021.

Latest revision as of 15:44, 5 October 2021


CDS_PKPa


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2059
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2059
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 598
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2059
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2059
    Illegal NgoMIV site found at 175
    Illegal NgoMIV site found at 2284
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of PKPa. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942 . In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases

Source

Coding sequence of this Xylulose-5-phosphate phosphoketolase has been found within the genome of Pseudomonas aeruginosa. Uniprot reference: https://www.uniprot.org/uniprot/A0A2R4KQ27

References

W. Xiong et al., "Phosphoketolase pathway contributes to carbon metabolism in cyanobacteria", 2021.

I. Bogorad, T. Lin and J. Liao, "Synthetic non-oxidative glycolysis enables complete carbon conservation", 2021.