Difference between revisions of "Part:BBa K3726001:Design"
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===Design Notes=== | ===Design Notes=== | ||
This part corresponds with the codon optimized coding sequence of PhaB^173S enzyme. | This part corresponds with the codon optimized coding sequence of PhaB^173S enzyme. | ||
− | Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>. | + | Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
− | "Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from | + | "Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from <i>Ralstoni eutropha</i> Reveals Two Mutations Responsible for Enhanced Kinetics | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/aem.01768-13?permanently=true. |
Latest revision as of 13:26, 5 October 2021
CDS_PhaB^173S
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part corresponds with the codon optimized coding sequence of PhaB^173S enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases
Source
Coding sequence of this Acetoacetyl-CoA reductase has been found within the genome of Ralstoni eutropha. Uniprot reference: https://www.uniprot.org/uniprot/P14697
References
"Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstoni eutropha Reveals Two Mutations Responsible for Enhanced Kinetics | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/aem.01768-13?permanently=true.