Difference between revisions of "Part:BBa K3726004:Design"
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This part corresponds with the codon optimized coding sequence of PduP enzyme. | This part corresponds with the codon optimized coding sequence of PduP enzyme. | ||
Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>. | Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>. | ||
| + | In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases | ||
===Source=== | ===Source=== | ||
Latest revision as of 13:09, 5 October 2021
CDS_PduP
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1246
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 252
Illegal NheI site found at 480
Illegal PstI site found at 1246 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1246
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1246
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part corresponds with the codon optimized coding sequence of PduP enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases
Source
Coding sequence of this Aldehyde dehydrogenase has been found within the genome of Salmonella enterica. Uniprot reference: https://www.uniprot.org/uniprot/A0A3V3TWW8
References
C. Fan et al., "Short N-terminal sequences package proteins into bacterial microcompartments", 2021.
C. Fan, S. Cheng, S. Sinha and T. Bobik, "Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments", 2021.