Difference between revisions of "Part:BBa K3726004:Design"

Latest revision as of 13:09, 5 October 2021

CDS_PduP

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1246
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 252
Illegal NheI site found at 480
Illegal PstI site found at 1246
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1246
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1246
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part corresponds with the codon optimized coding sequence of PduP enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases

Source

Coding sequence of this Aldehyde dehydrogenase has been found within the genome of Salmonella enterica. Uniprot reference: https://www.uniprot.org/uniprot/A0A3V3TWW8

References

C. Fan et al., "Short N-terminal sequences package proteins into bacterial microcompartments", 2021.

C. Fan, S. Cheng, S. Sinha and T. Bobik, "Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments", 2021.

@@ Line 8: / Line 8: @@
 ===Design Notes===
 This part corresponds with the codon optimized coding sequence of PduP enzyme.
-Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942.
+Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>.
+In addition the part sequence has been optimized to improve  mRNA stability removing internal recognition sites for endonucleases
 ===Source===