Difference between revisions of "Part:BBa K3726094"
 
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<partinfo>Bba_K3726094 short</partinfo>
 
<partinfo>Bba_K3726094 short</partinfo>
  
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The part is marburg collection designMoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.  
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The sequence of this parts corresponds with a rho-independent (intrinsec) terminator with a proved 99.9% terminating efficiency in UTEX 2973 either in optimal and suboptimal growth conditions.  
  
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This terminator part spans the 5A position of the MoClo syntax. The downstream overhang is GGTA, allowing the assembly of an additional expression cassette downstream the terminator part.
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The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.  
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This “5A” position terminator allows the introduction of an additional selection marker coupled to the transcriptional unit assembled during level 1 MoClo reaction. They are used for the creation of integrative genetic devices using Homology Connectors. To know more about, read the design principles of Marburg Collection. (Referencia a Marburg).
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<span class='h3bb'><b>Sequence & Features</b></span>
 
<span class='h3bb'><b>Sequence & Features</b></span>
 
<partinfo>BBa_K3726094 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3726094 SequenceAndFeatures</partinfo>
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Latest revision as of 17:23, 7 October 2021

T(5a)_Teck

The sequence of this parts corresponds with a rho-independent (intrinsec) terminator with a proved 99.9% terminating efficiency in UTEX 2973 either in optimal and suboptimal growth conditions.

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.


Sequence & Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]