Difference between revisions of "Part:BBa K3889000"
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{| class=wikitable | {| class=wikitable | ||
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| − | !Ex : Peak excitation wavelength (in | + | !Ex : Peak excitation wavelength (in nm) |
|383 | |383 | ||
|- | |- | ||
| − | !Em: Peak emission wavelength (in | + | !Em: Peak emission wavelength (in nm) |
|447 | |447 | ||
|- | |- | ||
| − | !EC: Extinction Coefficient (M-1 cm-1) | + | !EC: Extinction Coefficient (M<sup>-1</sup> cm<sup>-1</sup>) |
|26,200 | |26,200 | ||
|- | |- | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Azurite can be used to report protein production or in systems where fluorescence is required like FRET. In <partinfo>BBa_K3889101</partinfo> we planned to use Azurite as a reporter for P22 c2 repressor production (<partinfo>BBa_K3889101</partinfo>) in <i>Bacillus subtilis </i> 168. | ||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3889000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3889000 SequenceAndFeatures</partinfo> | ||
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| − | == | + | ==References== |
1. Mena, M. A., Treynor, T. P., Mayo, S. L., & Daugherty, P. S. (2006). Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Nature biotechnology, 24(12), 1569–1571. https://doi.org/10.1038/nbt1264 | 1. Mena, M. A., Treynor, T. P., Mayo, S. L., & Daugherty, P. S. (2006). Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Nature biotechnology, 24(12), 1569–1571. https://doi.org/10.1038/nbt1264 | ||
2. https://www.fpbase.org/protein/azurite/ | 2. https://www.fpbase.org/protein/azurite/ | ||
Latest revision as of 18:02, 19 October 2021
Azurite_BFP (BFP.B3)
Azurite is a Blue Fluorescence Reporter protein from Aequorea victoria and has the following parameters [1,2]:
| Ex : Peak excitation wavelength (in nm) | 383 |
|---|---|
| Em: Peak emission wavelength (in nm) | 447 |
| EC: Extinction Coefficient (M-1 cm-1) | 26,200 |
| QY: Quantum Yield | 0.55 |
| Brightness | 14.41 |
| pKa | 5.0 |
| Maturation (min) | 18.0 |
| Lifetime (ns) | 3.65 |
Usage and Biology
Azurite can be used to report protein production or in systems where fluorescence is required like FRET. In BBa_K3889101 we planned to use Azurite as a reporter for P22 c2 repressor production (BBa_K3889101) in Bacillus subtilis 168.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. Mena, M. A., Treynor, T. P., Mayo, S. L., & Daugherty, P. S. (2006). Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Nature biotechnology, 24(12), 1569–1571. https://doi.org/10.1038/nbt1264