Difference between revisions of "Part:BBa K3882001:Design"

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===Design Notes===
 
===Design Notes===
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Part1 equipped with LuxR to sense and combine the autoinducer, N-Acyl Homoserine Lactone (AHL) in the environment. After that, LuxR-AHL complex will be activated and bind to luxr-AHL response QS element to initiate the expression of mcherry report gene. T7 promoter serves as starting the whole synthetic gene. Lac operator is used for releasing LuxR. LuxR functions as receiving AHL signal molecules, and activates mCherry red fluorescence gene. QS promoter is the place that LuxR can recruit RNA polymerase. mCherry is a red fluorescence reporting gene.
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E. bsuahlscout bio-brick (BBa_K3882001) is design for detecting the AHL in the environment.
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QS promoter contains catabolite activator protein binding site and lux operator to enhance the transcription of PVDQ-GFP fusion protein.
  
 
===Source===
 
===Source===

Latest revision as of 03:30, 5 October 2021


E. bsuahlscout-QS-bsuahlteminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

E. bsuahlscout bio-brick (BBa_K3882001) is design for detecting the AHL in the environment.

QS promoter contains catabolite activator protein binding site and lux operator to enhance the transcription of PVDQ-GFP fusion protein.

Source

human

References