Difference between revisions of "Part:BBa K3875020"

 
 
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===Usage and Biology===
 
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The CRISPR targeting specificity is determined both by base pairing between the RNA chimera and the target DNA and by the binding between the Cas9 protein and a short DNA motif commonly found at the 3' end of the target DNA, called the protospacer adjacent motif (PAM). The N20 sequence is 5’-GGAATAACCGCTTCCCTCCC-3’. This sequence will help the Cas9 protein and gRNA complex to locate the fadR knockout site.<br>
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<img src="https://static.igem.org/mediawiki/parts/c/cc/T--BUCT--PCR_the_new_N20_sequence_in_Ptarget.png"width="640px";height="30px"/><br>
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Latest revision as of 08:02, 18 October 2021


Usage and Biology

The CRISPR targeting specificity is determined both by base pairing between the RNA chimera and the target DNA and by the binding between the Cas9 protein and a short DNA motif commonly found at the 3' end of the target DNA, called the protospacer adjacent motif (PAM). The N20 sequence is 5’-GGAATAACCGCTTCCCTCCC-3’. This sequence will help the Cas9 protein and gRNA complex to locate the fadR knockout site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]