Difference between revisions of "Part:BBa K3733034:Experience"

 
(→‎Applications of BBa_K3733034)
 
(3 intermediate revisions by 2 users not shown)
Line 5: Line 5:
  
 
===Applications of BBa_K3733034===
 
===Applications of BBa_K3733034===
 +
<p>
 +
We transform <i>E.coli</i> DH5α strain with BBa_K3733034 and chose neGFP (https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM, 10mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD<sub>600</sub> in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (<b>Figure 1</b>).
 +
</p>
 +
<html>
 +
<head>
 +
<meta charset="utf-8">
 +
<title>无标题文档</title>
 +
</head>
 +
<body>
 +
<center><img src="https://static.igem.org/mediawiki/parts/7/72/T--HZAU-China--105-1.png" style="width:784px;height:600px"></center>
 +
<center><b>Figure 1.</b>Comparison of OD<sub>600</sub> corrected RFU values under induction of different concentrations of thiosulfate </center>
 +
<br>
 +
</body>
 +
</html>
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 17:50, 17 October 2021


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3733034

We transform E.coli DH5α strain with BBa_K3733034 and chose neGFP (https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM, 10mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD600 in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).

无标题文档

Figure 1.Comparison of OD600 corrected RFU values under induction of different concentrations of thiosulfate

User Reviews

UNIQ06e32f81b821ea4e-partinfo-00000001-QINU UNIQ06e32f81b821ea4e-partinfo-00000002-QINU