Difference between revisions of "Part:BBa K3733033:Experience"

 
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===Applications of BBa_K3733033===
 
===Applications of BBa_K3733033===
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We transform <i>E.coli</i> DH5α strain with BBa_K3733033 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD<sub>600</sub> in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (<b>Figure 1</b>).
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<center><img src="https://static.igem.org/mediawiki/parts/c/c4/T--HZAU-China--104-1.png" style="width:784px;height:600px"></center>
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<center><b>Figure 1.</b>Comparison of OD<sub>600</sub> corrected RFU values under induction of different concentrations of thiosulfate </center>
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===User Reviews===
 
===User Reviews===

Latest revision as of 17:46, 17 October 2021


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Please enter how you used this part and how it worked out.

Applications of BBa_K3733033

We transform E.coli DH5α strain with BBa_K3733033 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD600 in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).

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Figure 1.Comparison of OD600 corrected RFU values under induction of different concentrations of thiosulfate

User Reviews

UNIQ0512c74fe894b768-partinfo-00000001-QINU UNIQ0512c74fe894b768-partinfo-00000002-QINU