Difference between revisions of "Part:BBa K3982006:Design"

 
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===Design Notes===
 
===Design Notes===
−
Restriction cloning
+
Restriction cloning using Xba1 and Spe1 RE.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
The part is derived from Addgene plasmid 112502
+
[https://www.addgene.org/112502/ Addgene plasmid 112502]
  
 
===References===
 
===References===

Latest revision as of 14:53, 21 October 2021


6xHis - Tagged Cas14a1 with TEV site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1411
    Illegal BamHI site found at 1005
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
    Illegal AgeI site found at 661
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction cloning using Xba1 and Spe1 RE.

Source

Addgene plasmid 112502

References