Difference between revisions of "Part:BBa K3887003"

 
 
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<partinfo>BBa_K3887003 short</partinfo>
 
<partinfo>BBa_K3887003 short</partinfo>
  
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Trp 5-halogenase, for production of dihalogenated indigos
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PyrH is another type of halogenase enzyme which shows regiospecificity at C7 during conversion of indole. In our project, it is used to biosynthesis other types of indigo using Trp 7-halogenase, similar to SttH producing Tyrian Purple. It is applied with the Fre-L3-halogenasee fusion technology. The Fre-L3-PyrH is placed in the TnaA action to convert to indole. Then, it is applied into the two-cell reaction system, with 2.5mM Trp as initiate substrate. As a result, several colors of halogenated indigos could be produced.
  
 
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Latest revision as of 03:14, 19 October 2021


PyrH

PyrH is another type of halogenase enzyme which shows regiospecificity at C7 during conversion of indole. In our project, it is used to biosynthesis other types of indigo using Trp 7-halogenase, similar to SttH producing Tyrian Purple. It is applied with the Fre-L3-halogenasee fusion technology. The Fre-L3-PyrH is placed in the TnaA action to convert to indole. Then, it is applied into the two-cell reaction system, with 2.5mM Trp as initiate substrate. As a result, several colors of halogenated indigos could be produced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1404
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 211
    Illegal NgoMIV site found at 829
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1384
    Illegal BsaI.rc site found at 1354
    Illegal SapI site found at 426