Difference between revisions of "Part:BBa K3896010"
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| − | 229E RBD | + | The target DNA can express a 121 amino acid protein, which is a segment of HCoV-229E RBD, including Lys201 to Ser321, and is a key part of binding to the receptor hAPN. The C-terminal has his tag, which is convenient for subsequent protein extraction and purification. The purified protein can specifically bind to pmrCAB (hAPN) to trigger downstream reaction. |
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Latest revision as of 15:39, 20 October 2021
HCoV-229E RBD with His-tag
The target DNA can express a 121 amino acid protein, which is a segment of HCoV-229E RBD, including Lys201 to Ser321, and is a key part of binding to the receptor hAPN. The C-terminal has his tag, which is convenient for subsequent protein extraction and purification. The purified protein can specifically bind to pmrCAB (hAPN) to trigger downstream reaction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 391
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 211
- 1000COMPATIBLE WITH RFC[1000]