Difference between revisions of "Part:BBa K3896009"

 
 
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<partinfo>BBa_K3896009 short</partinfo>
 
<partinfo>BBa_K3896009 short</partinfo>
  
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MERS RBD
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The target DNA can express a protein containing 110 amino acids, which is a segment of MERS-CoV RBD, including Gln471 to Asp580. It is a key part of binding to receptor DPP4. The C-terminal has his tag, which is convenient for subsequent protein extraction and purification. The purified protein can specifically bind to PmrCAB (DPP4) to trigger downstream reaction.
  
 
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Latest revision as of 08:32, 9 October 2021


MERS-CoV RBD with His-tag

The target DNA can express a protein containing 110 amino acids, which is a segment of MERS-CoV RBD, including Gln471 to Asp580. It is a key part of binding to receptor DPP4. The C-terminal has his tag, which is convenient for subsequent protein extraction and purification. The purified protein can specifically bind to PmrCAB (DPP4) to trigger downstream reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 295
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 358
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 295
  • 1000
    COMPATIBLE WITH RFC[1000]