Difference between revisions of "Part:BBa K3887002"
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− | + | tryptophan 7-halogenase | |
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+ | =Description= | ||
+ | RebH is a type of 7-halogenase enzyme used also during conversion of indole. | ||
+ | |||
+ | In our project, it is used to biosynthesis other types of indigo using Trp, similar to SttH producing Tyrian Purple. It is applied with the Fre-L3-halogenasee fusion technology. | ||
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Latest revision as of 07:52, 12 October 2021
RebH
tryptophan 7-halogenase
Description
RebH is a type of 7-halogenase enzyme used also during conversion of indole.
In our project, it is used to biosynthesis other types of indigo using Trp, similar to SttH producing Tyrian Purple. It is applied with the Fre-L3-halogenasee fusion technology.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 977
Illegal XhoI site found at 891
Illegal XhoI site found at 1537 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1423
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 958
Illegal BsaI.rc site found at 1540