Difference between revisions of "Part:BBa K3734010"

 
 
(7 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3734010 short</partinfo>
 
<partinfo>BBa_K3734010 short</partinfo>
  
 
TetR is a kind of protein that can find and combined with TRE DNA structure domain.
 
TetR is a kind of protein that can find and combined with TRE DNA structure domain.
 +
<html>
 +
 +
<head>
 +
   
 +
</head>
  
 +
<body>
 +
<h2 class="pageContent-main__title">
 +
                <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title-->
 +
                TetR
 +
            </h2>
 +
            <div class="pageContent-main__textBox">
 +
                <!--all the content must included in this div-->
 +
                <p>TetR can be identified and combined with TRE, which is an important part of the Tet-off system. In the absence of tetracycline, TetR binds to TRE.<br>When tetracycline exists,the binding of TetR and TRE will be blocked and the Tet-off system will be shut down.
 +
                </p>
 +
                <!--put text here, <p>content</p>-->
 +
            </div>
 +
            <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                <!--class="pageContent-main__title" means it is the sub title-->
 +
                1.Pattern diagram
 +
            </h2>
 +
            <div class="pageContent-main__textBox">
 +
                <!--all the content must included in this div-->
 +
                <p style="text-align: center;">
 +
                    <img width="400px" src="https://2021.igem.org/wiki/images/d/d1/T--CSU_CHINA--tupian13.png"></p>
 +
                <!--put image's url here-->
 +
                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram TetR-ELK</p>
 +
            </div>
 +
            <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                <!--class="pageContent-main__title" means it is the sub title-->
 +
                2.Experiment
 +
            </h2>
 +
            <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                <!--class="pageContent-main__title" means it is the sub title-->
 +
                2.1 Method
 +
            </h2>
 +
            <div class="pageContent-main__textBox">
 +
                <!--all the content must included in this div-->
 +
                <p>After TetR binds to TRE, phosphorylation ELK activates downstream reporting gene mCherry expression, and we observe the expression with laser confocal microscope.
 +
                </p>
 +
                <!--put text here, <p>content</p>-->
 +
            </div>
 +
            <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                <!--class="pageContent-main__title" means it is the sub title-->
 +
                2.2 Results
 +
            </h2>
 +
            <div class="pageContent-main__textBox">
 +
                <!--all the content must included in this div-->
 +
                <p style="text-align: center;">
 +
                    <img width="400px" src="https://2021.igem.org/wiki/images/5/54/T--CSU_CHINA--tupian14.png"></p>
 +
                <!--put image's url here-->
 +
                <br>
 +
                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system</p>
 +
            </div>
 +
            <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                <!--class="pageContent-main__title" means it is the sub title-->
 +
                3.Caution
 +
            </h2>
 +
            <div class="pageContent-main__textBox">
 +
                <!--all the content must included in this div-->
 +
                <p>If the green fluorescent protein is expressed too much, and if the green fluorescence is too strong, it may cause a series of colors. In order to ensure the accuracy of the experimental results, the level of noise reduction should be raised.
 +
                </p>
 +
            </div>
 +
            <h2 class="pageContent-main__title pageContent-main__subtitle">
 +
                <!--class="pageContent-main__title" means it is the sub title-->
 +
                Reference：
 +
            </h2>
 +
            <div class="pageContent-main__textBox">
 +
                <!--all the content must included in this div-->
 +
                <p>[1]Haifeng Ye, Mingqi Xie, Shuai Xue.Self-adjusting synthetic gene circuit for correcting insulin resistance[J].Nat Biomed Eng.2017 Jan;1(1):0005.
 +
                </p>
 +
            </div>
 +
</body>
 +
</html>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 15:13, 21 October 2021

TetR

TetR is a kind of protein that can find and combined with TRE DNA structure domain.

TetR

TetR can be identified and combined with TRE, which is an important part of the Tet-off system. In the absence of tetracycline, TetR binds to TRE.
When tetracycline exists,the binding of TetR and TRE will be blocked and the Tet-off system will be shut down.

1.Pattern diagram

Fig.1 The model diagram TetR-ELK

2.Experiment

2.1 Method

After TetR binds to TRE, phosphorylation ELK activates downstream reporting gene mCherry expression, and we observe the expression with laser confocal microscope.

2.2 Results


Fig.2 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system

3.Caution

If the green fluorescent protein is expressed too much, and if the green fluorescence is too strong, it may cause a series of colors. In order to ensure the accuracy of the experimental results, the level of noise reduction should be raised.

Reference：

[1]Haifeng Ye, Mingqi Xie, Shuai Xue.Self-adjusting synthetic gene circuit for correcting insulin resistance[J].Nat Biomed Eng.2017 Jan;1(1):0005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]