Difference between revisions of "Part:BBa K3798008"

 
 
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<partinfo>BBa_K3798008 short</partinfo>
 
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Silver is one of the most wasted precious metals that can be found in effluents, streams and even food. As our project is to develop a precious metal recycling platform, we chose Ag+ as the demonstration ion of this phase of the project. Aptamers are oligonucleotides that can bind targeted molecules with its secondary structures, in our project, Ag+ ion. BBa_K3798008 is a Ag+ aptamer obtained from a previous paper[1].
  
 
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<partinfo>BBa_K3798008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3798008 SequenceAndFeatures</partinfo>
  
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===Characterization===
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==Binding efficiency and stability==
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[[File:T--SHSBNU_China--silver ion 1.png|600px|thumb|center]]
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<p  class="figure-description"><b><center>Fig.1 Remaining Ag+ ion concentration of the solution after treated by different types of aptamers. </center></b></p >
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Aptamer 7 shows a binding efficacy of about 90%, although it seems like a high binding efficiency and satisfies the safe limit of concentration of the silver ions, it is not as stable nor efficient as Aptamer 4.  Therefore, this aptamer is discarded and was not subjected for further examination.
  
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===Reference===
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1. He, X., Qing, Z., Wang, K., Zou, Z., Shi, H., & Huang, J. (2012). Engineering a unimolecular multifunctional DNA probe for analysis of Hg2+ and Ag+. Analytical Methods, 4(2), 345. doi: 10.1039/c2ay05823e
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3798008 parameters</partinfo>
 
<partinfo>BBa_K3798008 parameters</partinfo>
 
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Latest revision as of 13:56, 19 October 2021


ssDNA, an aptamer for Ag+ ion

Silver is one of the most wasted precious metals that can be found in effluents, streams and even food. As our project is to develop a precious metal recycling platform, we chose Ag+ as the demonstration ion of this phase of the project. Aptamers are oligonucleotides that can bind targeted molecules with its secondary structures, in our project, Ag+ ion. BBa_K3798008 is a Ag+ aptamer obtained from a previous paper[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Binding efficiency and stability

T--SHSBNU China--silver ion 1.png

Fig.1 Remaining Ag+ ion concentration of the solution after treated by different types of aptamers.

Aptamer 7 shows a binding efficacy of about 90%, although it seems like a high binding efficiency and satisfies the safe limit of concentration of the silver ions, it is not as stable nor efficient as Aptamer 4. Therefore, this aptamer is discarded and was not subjected for further examination.

Reference

1. He, X., Qing, Z., Wang, K., Zou, Z., Shi, H., & Huang, J. (2012). Engineering a unimolecular multifunctional DNA probe for analysis of Hg2+ and Ag+. Analytical Methods, 4(2), 345. doi: 10.1039/c2ay05823e