Difference between revisions of "Part:BBa K3896003"

 
 
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<partinfo>BBa_K3896003 short</partinfo>
 
<partinfo>BBa_K3896003 short</partinfo>
  
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PmrCAB(DPP4)
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We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of DPP4 protein to be stimulated by the S protein of MERS-CoV. After detecting the S protein of MERS-CoV, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA. Then phosphorylated PmrA protein combined with promoter PmrC sequence to activate the expression of reporter gene.
  
 
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Latest revision as of 08:48, 9 October 2021


PmrCAB(DPP4)

We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of DPP4 protein to be stimulated by the S protein of MERS-CoV. After detecting the S protein of MERS-CoV, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA. Then phosphorylated PmrA protein combined with promoter PmrC sequence to activate the expression of reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 96
    Illegal NheI site found at 3043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3471
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1081
    Illegal AgeI site found at 2290
  • 1000
    COMPATIBLE WITH RFC[1000]