Difference between revisions of "Part:BBa K3734005"

 
 
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<partinfo>BBa_K3734005 short</partinfo>
 
<partinfo>BBa_K3734005 short</partinfo>
  
 
GAL4 is a kind of protein that can find and combined with UAS DNA structure domain.
 
GAL4 is a kind of protein that can find and combined with UAS DNA structure domain.
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                GAL4
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                <p>GAL4 is a common part in double yeast hybrid system. It can recognize and combine with UAS. Because GAL4 is originated from yeast cells, the activation domain can not work in mammalian cells. Therefore, we only used its combining domain
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                    and use it together with VP16 which is an activation domain comes from virus to realize the activation of downstream UAS transcription
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                1.Pattern diagram
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of GIP-miR21T-GI-GAL4-4XmiR21T</p>
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                2.Caution
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                <p>GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.
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                Reference：
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                <p>[1]Chun Jeih Ryu , Charles E Whitehurst, Jianzhu Chen.Expression of Gal4-VP16 and Gal4-DNA binding domain under the control of the T lymphocyte-specific lck proximal promoter in transgenic mice[J].BMB Rep. 2008 Aug 31;41(8):575-80.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:39, 20 October 2021

GAL4

GAL4 is a kind of protein that can find and combined with UAS DNA structure domain.

GAL4

GAL4 is a common part in double yeast hybrid system. It can recognize and combine with UAS. Because GAL4 is originated from yeast cells, the activation domain can not work in mammalian cells. Therefore, we only used its combining domain and use it together with VP16 which is an activation domain comes from virus to realize the activation of downstream UAS transcription

1.Pattern diagram

Fig.1 The model diagram of GIP-miR21T-GI-GAL4-4XmiR21T

2.Caution

GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.

Reference：

[1]Chun Jeih Ryu , Charles E Whitehurst, Jianzhu Chen.Expression of Gal4-VP16 and Gal4-DNA binding domain under the control of the T lymphocyte-specific lck proximal promoter in transgenic mice[J].BMB Rep. 2008 Aug 31;41(8):575-80.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 134