Difference between revisions of "Part:BBa K3888004:Design"
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<partinfo>BBa_K3888004 short</partinfo> | <partinfo>BBa_K3888004 short</partinfo> | ||
| − | + | [[File:pET28-pTac-lacO-hydrogenase .png]] | |
| + | ===Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.=== | ||
| + | HoxK1 encodes the small subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator. | ||
| + | <partinfo>BBa_K3888004 SequenceAndFeatures</partinfo> | ||
===Design Notes=== | ===Design Notes=== | ||
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===References=== | ===References=== | ||
| + | 1. Kim, Jaoon YH, Byung Hoon Jo, and Hyung Joon Cha. "Production of biohydrogen by heterologous expression of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli." Journal of biotechnology 155.3 (2011): 312-319. | ||
Latest revision as of 12:05, 20 October 2021
HoxK1
Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.
HoxK1 encodes the small subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 940
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Change signal sequence
Source
Hydrogenovibrio marinus MH-110 Genome
References
1. Kim, Jaoon YH, Byung Hoon Jo, and Hyung Joon Cha. "Production of biohydrogen by heterologous expression of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli." Journal of biotechnology 155.3 (2011): 312-319.