Difference between revisions of "Part:BBa K4060111"
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| − | a | + | To test how our kill switch design works, the expression of MazE and TetR can be evaluated by testing the RFP intensity under different L-arabinose concentrations and temperatures from Construct 2 & 3. We initially expected the bacteria with Construct 2 would show fluorescent red in LB medium (without L-arabinose). However, there was no red color in the colony of the bacteria, which means the RFP gene didn’t express. After using a photometer, the value of RFP intensity (RFP/OD600) is almost zero according to Fig.4, which is out of our expectation. |
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Latest revision as of 14:32, 7 December 2021
Const2
To test how our kill switch design works, the expression of MazE and TetR can be evaluated by testing the RFP intensity under different L-arabinose concentrations and temperatures from Construct 2 & 3. We initially expected the bacteria with Construct 2 would show fluorescent red in LB medium (without L-arabinose). However, there was no red color in the colony of the bacteria, which means the RFP gene didn’t express. After using a photometer, the value of RFP intensity (RFP/OD600) is almost zero according to Fig.4, which is out of our expectation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1661
Illegal AgeI site found at 1773 - 1000COMPATIBLE WITH RFC[1000]