Difference between revisions of "Part:BBa K3710004"
 
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This part encodes the Escherichia coli codon optimized parts for a Urea biosensor genetic circuit comprising the urea-specific transcription factor UreR and the bidirectional promoter of the Proteus mirabilis urease operon  pUreD.
 
  
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Proteus mirabilis encodes the transcriptional regulator UreR of the Urease gene cluster belonging to the AraC/XylS family of transcriptional regulators. UreR upregulates the gene clusters’ subunit transcription and recruits the accessory proteins (UreDABCEFG), when accompanied by urea, to form an active urease [2].
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__NOTOC__
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<partinfo>BBa_K3710004 short</partinfo>
  
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Pathogenic bacteria produce virulence factors such as ureases to assist in their colonisation efforts. Consequently, these ureases hydrolyse urea into NH3 and Carbonic acid, which increase local pH and provide a processed nitrogen source for growth. These changes assist the bacteria but also harm the host, causing mineral deposition on the urethral lumen resulting in urinary stones [1].
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This part harbours the urea biosensor composed of the urea-responsive transcriptional regulator UreR and the bidirectional promoter of the Proteus mirabilis urease operon PureD.
  
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pUreD is an inducible promoter that is activated by the UreR transcription factor in the presence of urea. pUreD is one of several promoters activated by the UreR transcriptional activator which share a consensus sequence of TA/GT/CA/TT/GC/TTA/TT/AATTG [3].  
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Proteus mirabilis encodes the transcriptional regulator UreR of the urease gene cluster. It belongs to the AraC/XylS family of transcriptional regulators. UreR upregulates the gene clusters’ subunit transcription in the presence of urea and recruits the accessory proteins (UreDABCEFG), forming an active urease [1].
  
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pUreD is a bidirectional promoter driving transcription of the UreR and UreD protein subunits of the urease operon of P. mirabilis. pUreD contains two ribosome binding sites and a UreR binding site about upstream of the forward transcriptional start site.  
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Pathogenic bacteria produce virulence factors, such as ureases, to assist in their colonisation efforts. Consequently, these ureases hydrolyse urea into NH3 and Carbonic acid, which increase local pH and provide a processed nitrogen source for growth. These changes assist the bacteria, but also harm the host, causing mineral deposition on the urethral lumen resulting in urinary stones [2].
  
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PureD is an inducible promoter that is activated by the UreR transcriptional regulator in the presence of urea. PureD is one of several promoters activated by the UreR transcriptional activator which share a consensus sequence of TA/GT/CA/TT/GC/TTA/TT/AATTG [3].
  
 
References:
 
References:
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[1] Poore, C. and Mobley, H. (2003) "Differential regulation of the Proteus mirabilis urease gene cluster by UreR and H-NS", Microbiology, 149(12), pp. 3383-3394. doi: 10.1099/mic.0.26624-0.
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[2] Dattelbaum, J. et al. (2003) "UreR, the Transcriptional Activator of the Proteus mirabilis Urease Gene Cluster, Is Required for Urease Activity and Virulence in Experimental Urinary Tract Infections", Infection and Immunity, 71(2), pp. 1026-1030. doi: 10.1128/iai.71.2.1026-1030.2003.
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[1] Poore, C. and Mobley, H. (2003) "Differential regulation of the Proteus mirabilis urease gene cluster by UreR and H-NS", Microbiology, 149(12), pp. 3383-3394.
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[2] Dattelbaum, J. et al. (2003) "UreR, the Transcriptional Activator of the Proteus mirabilis Urease Gene Cluster, Is Required for Urease Activity and Virulence in Experimental Urinary Tract Infections", Infection and Immunity, 71(2), pp. 1026-1030.  
  
 
[3]. Thomas V, Collins C. Identification of UreR binding sites in the Enterobacteriaceae plasmid-encoded and Proteus mirabilis urease gene operons. Molecular Microbiology. 1999;31(5):1417-1428.
 
[3]. Thomas V, Collins C. Identification of UreR binding sites in the Enterobacteriaceae plasmid-encoded and Proteus mirabilis urease gene operons. Molecular Microbiology. 1999;31(5):1417-1428.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3710004 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3710004 parameters</partinfo>
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<!-- -->

Latest revision as of 20:13, 21 October 2021


Urea Biosensor

This part harbours the urea biosensor composed of the urea-responsive transcriptional regulator UreR and the bidirectional promoter of the Proteus mirabilis urease operon PureD.

Proteus mirabilis encodes the transcriptional regulator UreR of the urease gene cluster. It belongs to the AraC/XylS family of transcriptional regulators. UreR upregulates the gene clusters’ subunit transcription in the presence of urea and recruits the accessory proteins (UreDABCEFG), forming an active urease [1].

Pathogenic bacteria produce virulence factors, such as ureases, to assist in their colonisation efforts. Consequently, these ureases hydrolyse urea into NH3 and Carbonic acid, which increase local pH and provide a processed nitrogen source for growth. These changes assist the bacteria, but also harm the host, causing mineral deposition on the urethral lumen resulting in urinary stones [2].

PureD is an inducible promoter that is activated by the UreR transcriptional regulator in the presence of urea. PureD is one of several promoters activated by the UreR transcriptional activator which share a consensus sequence of TA/GT/CA/TT/GC/TTA/TT/AATTG [3].

References:

[1] Poore, C. and Mobley, H. (2003) "Differential regulation of the Proteus mirabilis urease gene cluster by UreR and H-NS", Microbiology, 149(12), pp. 3383-3394.

[2] Dattelbaum, J. et al. (2003) "UreR, the Transcriptional Activator of the Proteus mirabilis Urease Gene Cluster, Is Required for Urease Activity and Virulence in Experimental Urinary Tract Infections", Infection and Immunity, 71(2), pp. 1026-1030.

[3]. Thomas V, Collins C. Identification of UreR binding sites in the Enterobacteriaceae plasmid-encoded and Proteus mirabilis urease gene operons. Molecular Microbiology. 1999;31(5):1417-1428.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1982
    Illegal AgeI site found at 2094
  • 1000
    COMPATIBLE WITH RFC[1000]