Difference between revisions of "Part:BBa K3794004"

 
 
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<partinfo>BBa_K3794004 short</partinfo>
 
<partinfo>BBa_K3794004 short</partinfo>
  
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ChABC Coding sequence.  
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Chondroitinase ABC (ChABC) is a bacterial enzyme secreted by <i> Proteus vulgaris </i>, it degrades the glycosaminoglycan (GAG) chains of chondroitin sulfate proteo-glycans (CSPGs) to allow for axonal regeneration following spinal cord injuries.  
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This ChABC part is derived from PDB ID: 1HN0, and also encodes a 6xHis tag and a TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation.
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ChABC can be administered in the form of microinjections to the glial scar that is formed during a spinal cord injury, to degrade GAG chains from CSPGs - which are inhibitory molecules to axonal regeneration.
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Due to the thermal instability of native ChABC at physiological temperatures, 8 point mutations to confer thermostability at 37C were introduced before synthesis of part in its composite form (BBa_K3794005) by IDT.
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These mutations are as follows:
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<ul>
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<li>K194E</li>
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<li>A228K</li>
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<li>S274P</li>
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<li>N288D</li>
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<li>S343N</li>
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<li>K654D</li>
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<li>R670T</li>
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<li>Q781E</li>
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</ul>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:21, 21 October 2021


ChABC CDS

Chondroitinase ABC (ChABC) is a bacterial enzyme secreted by Proteus vulgaris , it degrades the glycosaminoglycan (GAG) chains of chondroitin sulfate proteo-glycans (CSPGs) to allow for axonal regeneration following spinal cord injuries.

This ChABC part is derived from PDB ID: 1HN0, and also encodes a 6xHis tag and a TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation.

ChABC can be administered in the form of microinjections to the glial scar that is formed during a spinal cord injury, to degrade GAG chains from CSPGs - which are inhibitory molecules to axonal regeneration.

Due to the thermal instability of native ChABC at physiological temperatures, 8 point mutations to confer thermostability at 37C were introduced before synthesis of part in its composite form (BBa_K3794005) by IDT.

These mutations are as follows:

  • K194E
  • A228K
  • S274P
  • N288D
  • S343N
  • K654D
  • R670T
  • Q781E

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2754
  • 1000
    COMPATIBLE WITH RFC[1000]