Difference between revisions of "Part:BBa K3794000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Removed signal sequence from UniProt U5Y3S6. Codon optimised for expression in E.Coli K12 | + | Removed signal sequence from UniProt U5Y3S6. Codon optimised for expression in E.Coli K12. |
+ | |||
+ | Added N-terminal 6xHis tag with start codon and TEV cleavage site for protein purification via Ni-NTA column | ||
+ | |||
+ | Upstream of 6xHis tag, a start codon (ATG) was added to allow for transcriptional initiation, and a stop codon was added at 3' end of PVFP-5 CDS to allow for translational termination during protein synthesis | ||
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===Source=== | ===Source=== | ||
− | This part was synthesised by IDT. The | + | This part was synthesised as a composite by IDT (with an upstream LacI regulatory (BBa_R0010). The protein sequence was obtained from UniProtKB: U5Y3S6, a 6xHis tag and TEV cleavage site was added, and codon optimised for expression in E.Coli K12 |
===References=== | ===References=== |
Latest revision as of 17:01, 21 October 2021
PVFP-5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Removed signal sequence from UniProt U5Y3S6. Codon optimised for expression in E.Coli K12.
Added N-terminal 6xHis tag with start codon and TEV cleavage site for protein purification via Ni-NTA column
Upstream of 6xHis tag, a start codon (ATG) was added to allow for transcriptional initiation, and a stop codon was added at 3' end of PVFP-5 CDS to allow for translational termination during protein synthesis
Source
This part was synthesised as a composite by IDT (with an upstream LacI regulatory (BBa_R0010). The protein sequence was obtained from UniProtKB: U5Y3S6, a 6xHis tag and TEV cleavage site was added, and codon optimised for expression in E.Coli K12