Difference between revisions of "Part:BBa K4060110"

Latest revision as of 14:30, 7 December 2021

Const1

Since bioparts may behave differently in different host strains, any team who wish to integrate this kill switch system into their designs can use Construct 2 (BBa_K4060111) to roughly estimate MazE expression level in various condition. After that, a constitutive promoter with appropriate strength could be selected to construct a MazF constant expression system. Strength of constitutive promoters can be test out with an RFP reporter system, and we found that BBa_J23100 constitutive promoter family on BBa_J61002 reporter plasmid is useful for this purpose. A promoter that shows a slightly higher level of RFP expression in comparison with that of Construct 2 (BBa_K4060111) under L-arabinose induction (1mM) or low temperature (20℃) should be chosen. Integrate Construct 1 (BBa_K4060110) with the aforementioned MazF constant expression system in the same plasmid, and the kill switch system is completed.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1112
Illegal XbaI site found at 1128
Illegal SpeI site found at 1382
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1112
Illegal NheI site found at 125
Illegal SpeI site found at 1382
Illegal NotI site found at 1119
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1112
Illegal BamHI site found at 65
Illegal BamHI site found at 1179
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1112
Illegal XbaI site found at 1128
Illegal SpeI site found at 1382
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1112
Illegal XbaI site found at 1128
Illegal SpeI site found at 1382
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4060110 short</partinfo>
-a
+Since bioparts may behave differently in different host strains, any team who wish to integrate this kill switch system into their designs can use Construct 2 (BBa_K4060111) to roughly estimate MazE expression level in various condition.
+After that, a constitutive promoter with appropriate strength could be selected to construct a MazF constant expression system. Strength of constitutive promoters can be test out with an RFP reporter system, and we found that BBa_J23100 constitutive promoter family on BBa_J61002 reporter plasmid is useful for this purpose. A promoter that shows a slightly higher level of RFP expression in comparison with that of Construct 2 (BBa_K4060111) under L-arabinose induction (1mM) or low temperature (20℃) should be chosen. Integrate Construct 1 (BBa_K4060110) with the aforementioned MazF constant expression system in the same plasmid, and the kill switch system is completed.
 <!-- Add more about the biology of this part here