Difference between revisions of "Part:BBa K4060108"

 
 
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<partinfo>BBa_K4060108 short</partinfo>
 
<partinfo>BBa_K4060108 short</partinfo>
  
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BBa_K4060107 + BBa_K4060102
  
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The gene fragment Pre C1 is composed of Pre B4 and Pre A3. We digested the vector fragment Pre B4 with PstI and SpeI and the insert fragment Pre A3 with XbaI and PstI. Ee ligated these fragments together to form Pre C1.
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<html><body>
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<img src="https://2021.igem.org/wiki/images/4/4e/T--NYCU-Taipei--PreC1.png" height="475px" width="750px"/>
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</body></html>
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Agarose electrophoresis in order to verify Pre C1.
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Lane 1, 100bp ladderLane 2,3, Pre C1 digested with BamHI and HindIII.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 14:34, 7 December 2021


Pre C1

BBa_K4060107 + BBa_K4060102

The gene fragment Pre C1 is composed of Pre B4 and Pre A3. We digested the vector fragment Pre B4 with PstI and SpeI and the insert fragment Pre A3 with XbaI and PstI. Ee ligated these fragments together to form Pre C1.

Agarose electrophoresis in order to verify Pre C1. Lane 1, 100bp ladderLane 2,3, Pre C1 digested with BamHI and HindIII. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 145
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 754
    Illegal AgeI site found at 866
  • 1000
    COMPATIBLE WITH RFC[1000]