Difference between revisions of "Part:BBa K4060104"
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<partinfo>BBa_K4060104 short</partinfo> | <partinfo>BBa_K4060104 short</partinfo> | ||
| − | + | The gene fragment Pre B1 is composed of Pre A1 and the TetR gene <partinfo>BBa_C0040</partinfo>. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment <partinfo>BBa_C0040</partinfo> with XbaI and PstI. We ligated these fragments together to form Pre B1. | |
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Latest revision as of 14:26, 7 December 2021
[Pre B1] Pre A1 ligated before TetR
The gene fragment Pre B1 is composed of Pre A1 and the TetR gene BBa_C0040. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]